Development of the single-cell MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) mass-spectroscopic assay.

@article{Shimizu2003DevelopmentOT,
  title={Development of the single-cell MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) mass-spectroscopic assay.},
  author={M. Shimizu and N. Ojima and H. Ohnishi and T. Shingaki and Y. Hirakawa and T. Masujima},
  journal={Analytical sciences : the international journal of the Japan Society for Analytical Chemistry},
  year={2003},
  volume={19 1},
  pages={
          49-53
        }
}
  • M. Shimizu, N. Ojima, +3 authors T. Masujima
  • Published 2003
  • Chemistry, Medicine
  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
MALDI-TOF mass spectrometry was used to detect intracellular molecules from a single intact cell on monolayers of other cells. Intracellular molecules, e.g., histamine, were gradually increased in a mouse bone marrow-derived mast cell by a maturation process. A single cell was captured by a microsuction pipette, and the mass spectra of intracellular histamine were measured directly. Finally, the time course of the intracellular molecular contents and the maturation stage from a single cell were… Expand
17 Citations
Identification of mammalian cell lines using MALDI-TOF and LC-ESI-MS/MS mass spectrometry
  • 44
  • PDF
Molecular and functional analysis of cellular phenomena using single-cell mass spectrometry.
  • 16
Mass spectrometry for cellular and tissue analyses in a very small region.
  • N. Tsuyama, H. Mizuno, T. Masujima
  • Chemistry, Medicine
  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
  • 2011
  • 21
Recent advances in single-cell analysis by mass spectrometry.
  • 24
Analytical techniques for single-cell metabolomics: state of the art and trends
  • 117
  • PDF
Lab-on-a-plate: extending the functionality of MALDI-MS and LDI-MS targets.
  • 43
...
1
2
...

References

SHOWING 1-10 OF 18 REFERENCES
Trapping of secreted molecules at single-cell level using an HPLC resin particle under a videomicroscope.
  • 3
Induction of mast cell proliferation, maturation, and heparin synthesis by the rat c-kit ligand, stem cell factor.
  • M. Tsai, T. Takeishi, +5 authors S. Galli
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1991
  • 408
  • PDF
The kit ligand, stem cell factor.
  • 641
...
1
2
...