Development of substrate analogue inhibitors for the human airway trypsin-like protease HAT.

Abstract

A series of substrate analogue inhibitors of the serine protease HAT, containing a 4-amidinobenzylamide moiety as the P1 residue, was prepared. The most potent compounds possess a basic amino acid in the d-configuration as P3 residue. Whereas inhibitor 4 (K(i) 13 nM) containing proline as the P2 residue completely lacks selectivity, incorporation of norvaline leads to a potent inhibitor (15, K(i) 15 nM) with improved selectivity for HAT in comparison to the coagulation proteases thrombin and factor Xa or the fibrinolytic plasmin. Selected inhibitors were able to suppress influenza virus replication in a HAT-expressing MDCK cell model.

DOI: 10.1016/j.bmcl.2011.06.033

Cite this paper

@article{Sielaff2011DevelopmentOS, title={Development of substrate analogue inhibitors for the human airway trypsin-like protease HAT.}, author={Frank Sielaff and Eva B{\"{o}ttcher-Friebertsh{\"a}user and Daniela Meyer and Sebastian Martin Saupe and Ines M Volk and Wolfgang Garten and Torsten Steinmetzer}, journal={Bioorganic & medicinal chemistry letters}, year={2011}, volume={21 16}, pages={4860-4} }