Development of standardized approaches to reporting of minimal residual disease data using a reporting software package designed within the European LeukemiaNet

Abstract

Quantitative PCR (qPCR) for detection of fusion transcripts and overexpressed genes is a promising tool for following minimal residual disease (MRD) in patients with hematological malignancies. Its widespread clinical use has to some extent been hampered by differences in data analysis and presentation that complicate multicenter clinical trials. To address these issues, we designed a highly flexible MRD-reporting software program, in which data from various qPCR platforms can be imported, processed, and presented in a uniform manner to generate intuitively understandable reports. The software was tested in a two-step quality control (QC) study; the first step involved eight centers, whose previous experience with the software ranged from none to extensive. The participants received cDNA from consecutive samples from a BCR-ABL+ chronic myeloid leukemia (CML) patient and an acute myeloid leukemia (AML) patient with both CBFβ-MYH11 and WT1 target genes, they conducted qPCR on their respective hardware platforms and generated a series of reports with pre-defined features. In step two, five centers used the software to report BCR-ABL+ MRD in a harmonized manner, applying their recently obtained CML international scale conversion factors. The QC study demonstrated that this MRD-reporting software is suitable for efficient handling of qPCR data, generation of MRD reports and harmonization of MRD data.

DOI: 10.1038/leu.2011.69
01002002014201520162017
Citations per Year

123 Citations

Semantic Scholar estimates that this publication has 123 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{stergaard2011DevelopmentOS, title={Development of standardized approaches to reporting of minimal residual disease data using a reporting software package designed within the European LeukemiaNet}, author={Mette \Ostergaard and C G Nyvold and Jelena V. Jovanovic and M T Andersen and Veli Kairisto and Yvonne G Morgan and Khalid Tobal and Niels Pallisgaard and U {\"{O}zbek and Heike Pfeifer and Susanne Schnittger and Lykke Grubach and J\orgen K. Larsen and David J Grimwade and Peter Hokland}, journal={Leukemia}, year={2011}, volume={25}, pages={1168-1173} }