Development of specific antisera against 9-desglycinamide-8-arginine vasopressin by site-specific immunization.

@article{Bree1991DevelopmentOS,
  title={Development of specific antisera against 9-desglycinamide-8-arginine vasopressin by site-specific immunization.},
  author={J B van Bree and J W van Nispen and Joan A. C. Verhoef and D. D. Breimer},
  journal={Journal of pharmaceutical sciences},
  year={1991},
  volume={80 1},
  pages={46-9}
}
Four specific and sensitive antisera against 9-desglycinamide-8-arginine vasopressin (DGAVP) were obtained by means of site-specific immunization in rabbits, using a Keyhole Limpet haemocyanin-DGAVP conjugate synthesized by a specific reaction of a thiol function with a maleimide group. Compared with conventionally raised antisera, the cross-reactivity with endogenous arginine vasopressin (AVP) and vasopressin-derived fragments was substantially lower. Interassay variability was 18.1% at… CONTINUE READING