Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation.

@article{Nakagawa2007DevelopmentOS,
  title={Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation.},
  author={Tsuyoshi Nakagawa and Takayuki Kurose and Takeshi Hino and Katsunori Tanaka and Makoto Kawamukai and Yasuo Niwa and Kiminori Toyooka and Ken Matsuoka and Tetsuro Jinbo and Tetsuya Kimura},
  journal={Journal of bioscience and bioengineering},
  year={2007},
  volume={104 1},
  pages={34-41}
}
We developed a new series of binary vectors useful for Gateway cloning to facilitate transgenic experiments in plant biotechnology. The new system, Gateway Binary Vectors (pGWBs) realized efficient cloning, constitutive expression using the cauliflower mosaic virus (CaMV) 35S promoter and the construction of fusion genes by simple clonase reaction with an entry clone. The reporters employable in this system are beta-glucuronidase (GUS), synthetic green fluorescent protein with S65T mutation… CONTINUE READING
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