Development of selective, visible light-excitable, fluorescent magnesium ion probes with a novel fluorescence switching mechanism.

@article{Shoda2003DevelopmentOS,
  title={Development of selective, visible light-excitable, fluorescent magnesium ion probes with a novel fluorescence switching mechanism.},
  author={Takuji Shoda and Kazuya Kikuchi and Hirotatsu Kojima and Yasuteru Urano and Hirokazu Komatsu and Koji A. O. Suzuki and Tetsuo Nagano},
  journal={The Analyst},
  year={2003},
  volume={128 6},
  pages={719-23}
}
Novel fluorescent Mg2+ probes, 2'-carboxyfluorescein (2'-CF) and its derivatives, that are excitable by visible light, were designed, synthesized and characterized. After complexation with Mg2+, the absorption and emission maxima of these fluorescent probes were red-shifted and the fluorescence intensity was increased 11-fold at neutral pH. The apparent dissociation constant (Kd) of the Mg2+-2'-CF complex was 15.8 mM at neutral pH. The Kd of the Ca2+-2'-CF complex was about 10 times larger than… CONTINUE READING