Development of reliable detection assays for blueberry mosaic- and blackberry vein banding- associated viruses based on their population structures.

  title={Development of reliable detection assays for blueberry mosaic- and blackberry vein banding- associated viruses based on their population structures.},
  author={Thanuja Thekke-Veetil and Ioannis E. Tzanetakis},
  journal={Journal of virological methods},
Population structure, evolution and detection of blackberry leaf mottle‐associated virus, an emerging emaravirus
A quantitative PCR (qPCR) protocol was developed based on the knowledge obtained through the population structure of the virus; this is a sensitive test able to detect all the isolates studied and illustrated that the latter is at least 1000 times more sensitive.
Pest categorisation of non‐EU viruses of Rubus L.
A pest categorisation of 17 viruses of Rubus L. that were previously classified as either non‐EU or of undetermined standing in a previous opinion found that they do not meet the criteria to qualify as potential Union regulated non‐quarantine pests.
Epidemiological Studies of Soybean Vein Necrosis Virus and Potential Resistance Mechanisms to its Vector Neohydatothrips variabilis (Beach)
Research that aims to better understand virus epidemiology so as to develop effective virus control and disease management tools and to identify genotypes with resistance to the vector is presented.
Pest categorisation of non‐EU viruses of Ribes L.
These non‐EU viruses do not meet the criteria assessed by EFSA to qualify as potential Union regulated non‐quarantine pests, but tomato ringspot virus (ToRSV) has a wide host range, thus extending the possible entry pathways.
Is There a “Biological Desert” With the Discovery of New Plant Viruses? A Retrospective Analysis for New Fruit Tree Viruses
A baseline prioritization approach for publishing a new plant virus is proposed for proper assessment of the potential risks caused by a newly identified fruit tree virus.


Epidemiology of Blackberry yellow vein associated virus.
The information obtained in this study can be used at multiple levels to better understand and control blackberry yellow vein disease.
Detection of Strawberry necrotic shock virus using conventional and TaqMan(®) quantitative RT-PCR.
RT-PCR and Taqman(®) qPCR assays were developed based on conserved virus sequences identified in this isolate from Florida and other sequences for SNSV currently present in GenBank that applied successfully on multiple samples collected from several areas across the United States as well as isolates from around the world.
Identification, detection and transmission of a new vitivirus from Mentha
Sequence and phylogenetic analysis indicate that the virus identified in the atypical ‘Variegata’ clone, designated as mint virus-2, is related to members of the species Grapevine virus A, grapevine virus B and Heracleum latent virus, placing it in the genus Vitivirus.
Quasispecies Theory and the Behavior of RNA Viruses
Basic principles of quasispecies theory are discussed and its relevance for the understanding of viral fitness, virulence, and antiviral therapeutic strategy is described.
MUSCLE: multiple sequence alignment with high accuracy and high throughput.
  • R. Edgar
  • Biology, Medicine
    Nucleic acids research
  • 2004
MUSCLE is a new computer program for creating multiple alignments of protein sequences that includes fast distance estimation using kmer counting, progressive alignment using a new profile function the authors call the log-expectation score, and refinement using tree-dependent restricted partitioning.