Development of q-PCR approaches to assess water quality: effects of cadmium on gene expression of the diatom Eolimna minima.

@article{Tiam2012DevelopmentOQ,
  title={Development of q-PCR approaches to assess water quality: effects of cadmium on gene expression of the diatom Eolimna minima.},
  author={Sandra Kim Tiam and Agn{\`e}s Feurtet-Mazel and François Delmas and Nicolas Mazzella and Soizic Morin and Guillemine Daffe and Patrice Gonzalez},
  journal={Water research},
  year={2012},
  volume={46 4},
  pages={934-42}
}
This study was undertaken to develop molecular tools to assess water quality using diatoms as the biological model. Molecular approaches were designed following the development of a rapid and easy RNA extraction method suited to diatoms and the sequencing of genes involved in mitochondrial and photosystem metabolism. Secondly the impact of cadmium was evaluated at the genetic level by q-PCR on 9 genes of interest after exposure of Eolimna minima diatom populations cultured in suspension under… CONTINUE READING

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