Development of process to produce polyvalent IgY antibodies anti-African snake venom.

  title={Development of process to produce polyvalent IgY antibodies anti-African snake venom.},
  author={Cl{\'a}udia Maria Costa de Almeida and Cl{\'a}udia Let{\'i}cia da Silva and Humberto Pena Couto and Rita de C{\'a}ssia Moth{\'e} Escocard and David Gitirana da Rocha and Lynna de Paula Sentinelli and Thereza Liberman Kipnis and Wilmar Dias da Silva},
  journal={Toxicon : official journal of the International Society on Toxinology},
  volume={52 2},
Polyvalent anti-Bitis and anti-Naja antivenom IgY antibodies were prepared using B. arietans, B. nasicornis, B. rhinoceros, N. melanoleuca, and N. mossambica venoms to immunize chickens. Blood and eggs were collected before and during the 10-month immunization period; the sera and yolk extracts were then prepared and assayed for the presence of antivenom antibodies by ELISA and Western blot methods. ELISA Antivenom antibody titers, referred to as U-ELISA/ml of serum or egg yolk extracts, absent… CONTINUE READING
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