Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors.

@article{Jiang2005DevelopmentOP,
  title={Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors.},
  author={Jianlin Jiang and Kerri A Alderisio and Ajaib Singh and Lihua Xiao},
  journal={Applied and environmental microbiology},
  year={2005},
  volume={71 3},
  pages={1135-41}
}
Extraction of high-quality DNA is a key step in PCR detection of Cryptosporidium and other pathogens in environmental samples. Currently, Cryptosporidium oocysts in water samples have to be purified from water concentrates before DNA is extracted. This study compared the effectiveness of six DNA extraction methods (DNA extraction with the QIAamp DNA minikit… CONTINUE READING