Development of primary cell culture from Scylla serrata

@article{Sashikumar2008DevelopmentOP,
  title={Development of primary cell culture from Scylla serrata},
  author={Anu Sashikumar and Prashant V. Desai},
  journal={Cytotechnology},
  year={2008},
  volume={56},
  pages={161-169}
}
This paper reports for the first time, the Primary cell culture of hepatopancreas from edible crab Scylla serrata using crab saline, L-15 (Leibovitz), 1 × L-15 + crab saline, 2 × L-15 + crab saline, 3 × L-15 and citrate buffer without any serum. We could isolate and maintain E (Embryonalzellen), F (Fibrenzellen), B (Blasenzellen), R (Restzellen) and G (Granular cells). Upon seeding the hepatopancreatic E, F, B, and R cells showed different survival pattern over time than granular cells. A… CONTINUE READING

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