Development of porcine transgenic nuclear-transferred embryos derived from fibroblast cells transfected by the novel technique of nucleofection or standard lipofection.

@article{Skrzyszowska2008DevelopmentOP,
  title={Development of porcine transgenic nuclear-transferred embryos derived from fibroblast cells transfected by the novel technique of nucleofection or standard lipofection.},
  author={Maria Skrzyszowska and Marcin Samiec and Ryszard Słomski and Daniel Lipinski and Ewa Mały},
  journal={Theriogenology},
  year={2008},
  volume={70 2},
  pages={248-59}
}
The aim of our study was to determine the in vitro developmental potential of porcine nuclear-transferred (NT) embryos that had been reconstructed with Tg(pWAPhGH-GFPBsd) transgene-expressing fibroblast cells. The gene construct was introduced into fibroblast cells by the novel method of nucleofection or standard lipofection. NT oocytes derived from foetal… CONTINUE READING