Development of polymorphic nuclear microsatellite markers in sugar maple (Acer saccharum Marsh.) using cross-species transfer and SSR-enriched shotgun pyrosequencing

@article{Graignic2013DevelopmentOP,
  title={Development of polymorphic nuclear microsatellite markers in sugar maple (Acer saccharum Marsh.) using cross-species transfer and SSR-enriched shotgun pyrosequencing},
  author={No{\'e}mie Graignic and Francine M. Tremblay and Yves Bergeron},
  journal={Conservation Genetics Resources},
  year={2013},
  volume={5},
  pages={845-848}
}
A set of 23 polymorphic microsatellites was successfully tested for Acer saccharum. Eighteen of these new polymorphic markers were developed using next-generation sequencing and five were transferred from other Acer species. A total of 68 individuals from two populations were genotyped at all markers using a multiplex approach. Mean allelic diversity was 7.7, polymorphism ranged from 2 to 16 alleles per locus. Overall heterozygosity was high, with an average observed heterozygosity of 0.644… CONTINUE READING

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