Development of pLR591, a Streptomyces-Escherichia coli positive selection shuttle vector.

Abstract

The Escherichia coli positive selection vector pEcoR251 was ligated with the broad host range, high copy number Streptomyces plasmid pIJ702 to produce pLR591, a Streptomyces-E. coli positive selection shuttle vector. The EcoRI and thiostrepton resistance genes of pLR591 were expressed in E. coli and Streptomyces lividans respectively. The positive selection… (More)

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@article{Hill1989DevelopmentOP, title={Development of pLR591, a Streptomyces-Escherichia coli positive selection shuttle vector.}, author={Ronald T. Hill and Nicola Illing and Roy Kirby and David Woods}, journal={FEMS microbiology letters}, year={1989}, volume={48 2}, pages={223-6} }