Development of oxidized phosphatidylcholine isomer profiling method using supercritical fluid chromatography/tandem mass spectrometry.

@article{Uchikata2012DevelopmentOO,
  title={Development of oxidized phosphatidylcholine isomer profiling method using supercritical fluid chromatography/tandem mass spectrometry.},
  author={Takato Uchikata and Atsuki Matsubara and Shin Nishiumi and Masaru Yoshida and Eiichiro Fukusaki and Takeshi Bamba},
  journal={Journal of chromatography. A},
  year={2012},
  volume={1250},
  pages={205-11}
}
Phospholipids that contain polyunsaturated fatty acid are easily oxidized by free radicals or oxidants and can yield numerous oxidation species, including positional and structural isomers. However, it is difficult to separate these oxidation products for structural analysis. In this study, a high-resolution separation analytical system based on supercritical fluid chromatography with tandem mass spectrometry (SFC/MS/MS) was established for the separation and identification of oxidized… CONTINUE READING
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