Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR

@inproceedings{Zhang2011DevelopmentOO,
  title={Development of one-step SYBR Green real-time RT-PCR for quantifying bovine viral diarrhea virus type-1 and its comparison with conventional RT-PCR},
  author={Ni Zhang and Zhengwen Liu and Qunying Han and Jianming Qiu and Jinghong Chen and Guoyu Zhang and Zhu Li and Sai Lou and Na Li},
  booktitle={Virology Journal},
  year={2011}
}
Bovine viral diarrhea virus (BVDV) is a worldwide pathogen in cattle and acts as a surrogate model for hepatitis C virus (HCV). One-step real-time fluorogenic quantitative reverse transcription polymerase chain reaction (RT-PCR) assay based on SYBR Green I dye has not been established for BVDV detection. This study aims to develop a quantitative one-step RT-PCR assay to detect BVDV type-1 in cell culture. One-step quantitative SYBR Green I RT-PCR was developed by amplifying cDNA template from… CONTINUE READING

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