Development of one-step, real-time, quantitative reverse transcriptase PCR assays for absolute quantitation of human coronaviruses OC43 and 229E.

@article{Vijgen2005DevelopmentOO,
  title={Development of one-step, real-time, quantitative reverse transcriptase PCR assays for absolute quantitation of human coronaviruses OC43 and 229E.},
  author={Leen Vijgen and Els Keyaerts and Elien Mo{\"e}s and Piet Maes and Griet Duson and Marc van Ranst},
  journal={Journal of clinical microbiology},
  year={2005},
  volume={43 11},
  pages={5452-6}
}
The clinical significance of human coronaviruses in more severe respiratory illnesses has recently been shown to be higher than was previously assumed. Rapid and reliable diagnosis of human coronavirus infections therefore becomes indispensable in a routine clinical setting. In this study, we present a very sensitive and specific TaqMan-based, real-time quantitative reverse transcriptase PCR (qRT-PCR) for the rapid detection and quantitation of human coronaviruses (HCoVs) OC43 and 229E… CONTINUE READING
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