Development of novel yeast cell surface display system for homo-oligomeric protein by coexpression of native and anchored subunits.

@article{Furukawa2006DevelopmentON,
  title={Development of novel yeast cell surface display system for homo-oligomeric protein by coexpression of native and anchored subunits.},
  author={Hirotaka Furukawa and Takanori Tanino and Hideki Fukuda and Akihiko Kondo},
  journal={Biotechnology progress},
  year={2006},
  volume={22 4},
  pages={994-7}
}
Streptavidin derived from Streptomyces avidinii was displayed on the cell surface of the yeast Saccharomyces cerevisiae by cell-surface engineering using two types of plasmid for the expression of a native subunit and an anchored subunit fused with the C-terminus of 318 amino acids of Flo1p containing a glycosylphosphatidylinositol anchor attachment signal. The displayed streptavidin had the binding ability for biotinylated compounds. This was confirmed by fluorescence microscopy after the… CONTINUE READING

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