Development of novel FP-based probes for live-cell imaging of nitric oxide dynamics

@inproceedings{Eroglu2016DevelopmentON,
  title={Development of novel FP-based probes for live-cell imaging of nitric oxide dynamics},
  author={Emrah Eroglu and Benjamin Gottschalk and Suphachai Charoensin and Sandra Blass and Helmut Bischof and Rene Rost and Corina T Madreiter-Sokolowski and Brigitte Pelzmann and Eva Bernhart and Wolfgang Sattler and Seth Hallstroem and Tadeusz Malinski and Markus Waldeck-Weiermair and Wolfgang F. Graier and Roland Malli},
  booktitle={Nature communications},
  year={2016}
}
  • Emrah Eroglu, Benjamin Gottschalk, +12 authors Roland Malli
  • Published in Nature communications 2016
  • Medicine, Biology
  • Nitric oxide () is a free radical with a wide range of biological effects, but practically impossible to visualize in single cells. Here we report the development of novel multicoloured fluorescent quenching-based probes by fusing a bacteria-derived -binding domain close to distinct fluorescent protein variants. These genetically encoded probes, referred to as geNOps, provide a selective, specific and real-time read-out of cellular dynamics and, hence, open a new era of bioimaging. The… CONTINUE READING

    Create an AI-powered research feed to stay up to date with new papers like this posted to ArXiv

    Figures and Topics from this paper.

    Citations

    Publications citing this paper.
    SHOWING 1-10 OF 23 CITATIONS

    Genetic biosensors for imaging nitric oxide in single cells☆

    VIEW 10 EXCERPTS
    CITES METHODS & BACKGROUND

    Formation of Nitric Oxide by Aldehyde Dehydrogenase-2 Is Necessary and Sufficient for Vascular Bioactivation of Nitroglycerin*

    VIEW 9 EXCERPTS
    CITES METHODS & BACKGROUND

    References

    Publications referenced by this paper.
    SHOWING 1-10 OF 54 REFERENCES

    Regulation of the mitochondrial proton gradient by cytosolic Ca2+ signals

    Nitric oxide physiology and pathology.