Development of novel FP-based probes for live-cell imaging of nitric oxide dynamics

@inproceedings{Eroglu2016DevelopmentON,
  title={Development of novel FP-based probes for live-cell imaging of nitric oxide dynamics},
  author={Emrah Eroglu and Benjamin Gottschalk and Suphachai Charoensin and Sandra Blass and Helmut Bischof and Rene Rost and Corina T Madreiter-Sokolowski and Brigitte Pelzmann and Eva Maria Bernhart and Wolfgang Sattler and Seth Hallstroem and Tadeusz Malinski and Markus Waldeck-Weiermair and Wolfgang F. Graier and Roland Malli},
  booktitle={Nature communications},
  year={2016}
}
Nitric oxide () is a free radical with a wide range of biological effects, but practically impossible to visualize in single cells. Here we report the development of novel multicoloured fluorescent quenching-based probes by fusing a bacteria-derived -binding domain close to distinct fluorescent protein variants. These genetically encoded probes, referred to as geNOps, provide a selective, specific and real-time read-out of cellular dynamics and, hence, open a new era of bioimaging. The… CONTINUE READING
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