Development of normal mice from metaphase I oocytes fertilized with primary spermatocytes.

  title={Development of normal mice from metaphase I oocytes fertilized with primary spermatocytes.},
  author={Atsuo Ogura and Osamu Suzuki and Kentaro Tanemura and Keiji Mochida and Yasuhiro Kobayashi and Jun-ichi Matsuda},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={95 10},
Primary spermatocytes are the male germ cells before meiosis I. To examine whether these 4n diploid cells are genetically competent to fertilize oocytes and support full embryo development, we introduced the nuclei of pachytene/diplotene spermatocytes into oocytes that were arrested in prophase I (germinal vesicle stage), metaphase I, or metaphase II (Met II). Both the paternal and maternal chromosomes then were allowed to undergo meiosis synchronously until Met II. In the first and second… CONTINUE READING


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Ogura et al

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