Development of new potato virus X-based vectors for gene over-expression and gene silencing assay.

@article{Wang2014DevelopmentON,
  title={Development of new potato virus X-based vectors for gene over-expression and gene silencing assay.},
  author={Ying Wang and Qian-Qian Cong and Y. Lan and Chao Geng and Xian-Dao Li and Yuan-Cun Liang and Z Yang and Xiao-ping Zhu and Xiang-Dong Li},
  journal={Virus research},
  year={2014},
  volume={191},
  pages={62-9}
}
Multiple plant viruses, including potato virus X (PVX), have been modified as vectors for expressing heterologous genes or silencing endogenous genes in plants. PVX-based vectors facilitate the functional analysis of genes in plant. However, they can only express one protein in a time. In this paper we report the construction of new vectors based on a 35S promoter-driven PVX infectious clone, pCaPVX100. Vector pCaPVX440 contains two additional subgenomic promoters and can be utilized to express… CONTINUE READING