Development of nested PCR assays for detection of bovine respiratory syncytial virus in clinical samples.

@article{Vilek1994DevelopmentON,
  title={Development of nested PCR assays for detection of bovine respiratory syncytial virus in clinical samples.},
  author={{\vS}tefan Vil{\vc}ek and Marianne Elvander and Andr{\'a}s Ballagi-Pord{\'a}ny and S{\'a}nd{\'o}r Bel{\'a}k},
  journal={Journal of clinical microbiology},
  year={1994},
  volume={32 9},
  pages={2225-31}
}
Two nested PCR assays were developed for the detection of bovine respiratory syncytial virus (BRSV). Primers were selected from the gene encoding the F fusion protein (PCR-F) and the gene encoding the G attachment protein (PCR-G). Biotinylated oligonucleotide probes, termed F and G, were selected for the hybridization of the respective PCR products. The sensitivities of the PCR-F and PCR-G assays were similar, both detecting 0.1 tissue culture infective dose of the virus. The PCR-F assay… CONTINUE READING
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