Development of multiplex polymerase chain reaction for detection of Ehrlichia canis, Babesia spp and Hepatozoon canis in canine blood.

@article{Kledmanee2009DevelopmentOM,
  title={Development of multiplex polymerase chain reaction for detection of Ehrlichia canis, Babesia spp and Hepatozoon canis in canine blood.},
  author={Kan Kledmanee and Sarin Suwanpakdee and Sakranmanee Krajangwong and Jarin Chatsiriwech and Parut Suksai and Pongpun Suwannachat and Ladawan Sariya and Ruangrat Buddhirongawatr and Phingphol Charoonrut and Kridsada Chaichoun},
  journal={The Southeast Asian journal of tropical medicine and public health},
  year={2009},
  volume={40 1},
  pages={35-9}
}
A multiplex polymerase chain reaction (PCR) has been developed for simultaneous detection of canine blood parasites, Ehrlichia canis, Babesia spp and Hepatozoon canis, from blood samples in a single reaction. The multiplex PCR primers were specific to E. canis VirB9, Babesia spp 16S rRNA and H. canis 16S rRNA genes. Specificity of the amplicons was confirmed by DNA sequencing. The assay was evaluated using normal canine and infected blood samples, which were detected by microscopic examination… CONTINUE READING

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