Development of monoclonal antibodies against chicken IgM and its application in immunity studies

Immunoglobulin M (IgM) from specific pathogen free (SPF) chicken serum was purified and used for production of monoclonal antibodies (MAbs) for subsequent application in the detection of primary immune response to infectious agents. Chicken IgM was fractionated by gel filtration technique using Sephadex G-200. Purity of the fractionated IgM was confirmed by… CONTINUE READING