Development of microsatellite markers in Fosterella rusbyi (Bromeliaceae) using 454 pyrosequencing.

@article{Whrmann2012DevelopmentOM,
  title={Development of microsatellite markers in Fosterella rusbyi (Bromeliaceae) using 454 pyrosequencing.},
  author={Tina W{\"o}hrmann and Natascha Dorothea Wagner and Florian Krapp and Bruno Huettel and Kurt Weising},
  journal={American journal of botany},
  year={2012},
  volume={99 4},
  pages={
          e160-3
        }
}
PREMISE OF THE STUDY Polymorphic microsatellite markers were developed for Fosterella rusbyi (Bromeliaceae) to evaluate the population genetic structure and genetic diversity of natural populations of F. rusbyi and other Fosterella species in Bolivia. METHODS AND RESULTS 454 pyrosequencing technology was used to generate 73027 sequence reads from F. rusbyi DNA, which together contained 2796 perfect simple sequence repeats (SSRs). Primer pairs were designed for 30 loci, of which 15 were used… Expand
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