Development of microsatellite markers in Fosterella rusbyi (Bromeliaceae) using 454 pyrosequencing.

@article{Whrmann2012DevelopmentOM,
  title={Development of microsatellite markers in Fosterella rusbyi (Bromeliaceae) using 454 pyrosequencing.},
  author={Tina W{\"o}hrmann and Natascha Wagner and Florian Krapp and B. Huettel and K. Weising},
  journal={American journal of botany},
  year={2012},
  volume={99 4},
  pages={
          e160-3
        }
}
  • Tina Wöhrmann, Natascha Wagner, +2 authors K. Weising
  • Published 2012
  • Biology, Medicine
  • American journal of botany
  • PREMISE OF THE STUDY Polymorphic microsatellite markers were developed for Fosterella rusbyi (Bromeliaceae) to evaluate the population genetic structure and genetic diversity of natural populations of F. rusbyi and other Fosterella species in Bolivia. METHODS AND RESULTS 454 pyrosequencing technology was used to generate 73027 sequence reads from F. rusbyi DNA, which together contained 2796 perfect simple sequence repeats (SSRs). Primer pairs were designed for 30 loci, of which 15 were used… CONTINUE READING
    20 Citations

    Topics from this paper.

    Development of 18 polymorphic microsatellite markers for Vinca minor (Apocynaceae) via 454 pyrosequencing1
    Reproduction biology and chloroplast inheritance in Bromeliaceae: a case study in Fosterella (Pitcairnioideae)
    • 17

    References

    SHOWING 1-8 OF 8 REFERENCES
    Phylogenetic analysis of Fosterella L.B. Sm. (Pitcairnioideae, Bromeliaceae) based on four chloroplast DNA regions.
    • 31
    • PDF
    Genic microsatellite markers in plants: features and applications.
    • 1,517
    • PDF
    BatchPrimer3: A high throughput web application for PCR and sequencing primer design
    • 604
    • PDF
    Arlequin (version 3.0): An integrated software package for population genetics data analysis
    • 13,203
    • PDF