Development of methotrexate resistance in a human squamous cell carcinoma of the head and neck in culture.

@article{Frei1984DevelopmentOM,
  title={Development of methotrexate resistance in a human squamous cell carcinoma of the head and neck in culture.},
  author={Emil Frei and Andre Rosowsky and Joel E. Wright and Costanza Cucchi and Judith A Lippke and Thomas J. Ervin and Jacques Jolivet and William A. Haseltine},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1984},
  volume={81 9},
  pages={2873-7}
}
Four methotrexate (MTX)-resistant sublines of a human squamous cell carcinoma (SCC15) were established in culture by progressive dose escalation. The biochemical basis of resistance was studied. The line with the lowest resistance (R1) had a normal dihydrofolate reductase (DHFR) content but showed decreased MTX transport and polyglutamation. Lines of intermediate resistance (R2 and R3) showed an increased DHFR content and DHFR gene copy number and a defect in MTX transport. The line with the… CONTINUE READING

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