Development of metagenomic DNA shuffling for the construction of a xenobiotic gene.

@article{Boubakri2006DevelopmentOM,
  title={Development of metagenomic DNA shuffling for the construction of a xenobiotic gene.},
  author={Hasna Boubakri and M{\'e}lanie Beuf and Pascal Simonet and Timothy M. Vogel},
  journal={Gene},
  year={2006},
  volume={375},
  pages={87-94}
}
We describe a metagenomic DNA shuffling process by combining protein engineering process mutation generator and the high potential diversity of metagenomic DNA derived from the environment. Numerous previous shuffling processes attempted to recombine more or less related parental sequences. At the same time, metagenomic approaches unveiled a huge diversity of DNA sequences and genomes, which have not yet been identified to date. In this study, we attempted to combine these two approaches in… CONTINUE READING

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