Development of melting temperature-based SYBR Green I polymerase chain reaction methods for multiplex genetically modified organism detection.

@article{Hernndez2003DevelopmentOM,
  title={Development of melting temperature-based SYBR Green I polymerase chain reaction methods for multiplex genetically modified organism detection.},
  author={Marta Hern{\'a}ndez and David Rodr{\'i}guez-L{\'a}zaro and Teresa Valverde Esteve and Salom{\'e} Prat and Maria Pla},
  journal={Analytical biochemistry},
  year={2003},
  volume={323 2},
  pages={164-70}
}
Commercialization of several genetically modified crops has been approved worldwide to date. Uniplex polymerase chain reaction (PCR)-based methods to identify these different insertion events have been developed, but their use in the analysis of all commercially available genetically modified organisms (GMOs) is becoming progressively insufficient. These… CONTINUE READING