Development of mature BDNF-specific sandwich ELISA.

  title={Development of mature BDNF-specific sandwich ELISA.},
  author={Yoon Lim and Jin-Hua Zhong and Xin-fu Zhou},
  journal={Journal of neurochemistry},
  volume={134 1},
Mature brain-derived neurotrophic factor (mBDNF) plays a vital role in the nervous system, whereas proBDNF elicits neurodegeneration and neuronal apoptosis. Although current enzyme-linked immunosorbent assay (ELISA) has been widely used to measure BDNF levels, it cannot differentiate mBDNF from proBDNF. As the function of proBDNF differs from mBDNF, it is necessary to establish an ELISA assay specific for the detection of mBDNF. Therefore, we aimed to establish a new mBDNF-specific sandwich… CONTINUE READING
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