Development of mature BDNF‐specific sandwich ELISA

@article{Lim2015DevelopmentOM,
  title={Development of mature BDNF‐specific sandwich ELISA},
  author={Yoon Lim and Jin-hua Zhong and Xin-Fu Zhou},
  journal={Journal of Neurochemistry},
  year={2015},
  volume={134}
}
Mature brain‐derived neurotrophic factor (mBDNF) plays a vital role in the nervous system, whereas proBDNF elicits neurodegeneration and neuronal apoptosis. Although current enzyme‐linked immunosorbent assay (ELISA) has been widely used to measure BDNF levels, it cannot differentiate mBDNF from proBDNF. As the function of proBDNF differs from mBDNF, it is necessary to establish an ELISA assay specific for the detection of mBDNF. Therefore, we aimed to establish a new mBDNF‐specific sandwich… 
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TLDR
This work was supported by grants from the Applied Basic Research Foundation of Yunnan Province (CN) 2017FE468(-101), the Medical and Health Science and Technology Project of Kunming Health Committee (2018-03-09-001), and the Graduate Innovation Fund Project ofkunming Medical University (2019S062).
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It is shown that proBDNF and pan neurotrophic receptor p75 were significantly upregulated in monocytes from healthy donors (HD) after lipopolysaccharide treatment, suggesting that upregulation of proBD NF in M2‐like monocytes may contribute to the proinflammatory response in the AAD.
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