Development of mRNA-specific RT-PCR for the detection of koi herpesvirus (KHV) replication stage.

@article{Yuasa2012DevelopmentOM,
  title={Development of mRNA-specific RT-PCR for the detection of koi herpesvirus (KHV) replication stage.},
  author={Kei Yuasa and Jun Kurita and Morihiko Kawana and Ikunari Kiryu and Norihisa Oseko and Motohiko Sano},
  journal={Diseases of aquatic organisms},
  year={2012},
  volume={100 1},
  pages={11-8}
}
An mRNA-specific reverse transcription (RT)-PCR primer set spanning the exon junction of a spliced putative terminase gene in the koi herpesvirus (KHV) was developed to detect the replicating stage of the virus. The proposed RT-PCR amplified a target gene from the RNA template, but not from a DNA template extracted from common carp brain (CCB) cells infected with KHV. In addition, the RT-PCR did not amplify the target gene of templates extracted from specific cell lines infected with either… CONTINUE READING
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References

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Improvement of a PCR method with the Sph 1 - 5 primer set for the detection of koi herpesvirus ( KHV )

  • K Yuasa, M Sano, J Kurita, T Ito, T Iida
  • Fish Pathol
  • 2005
Highly Influential
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Genome sequences of two frog herpesviruses

  • C Cunningham, W Sauerbier
  • 2006

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