Development of loop-mediated isothermal amplification method for detection of Akabane disease virus

@inproceedings{Jihon2013DevelopmentOL,
  title={Development of loop-mediated isothermal amplification method for detection of Akabane disease virus},
  author={Zhang Ji-hon},
  year={2013}
}
In the present study,a loop-mediated isothermal amplification(LAMP) method for detection of Akabane virus was developed using a set of primers designed according to the S gene of the virus.Under the optimized reaction conditions,the LAMP was specific to amplified the DNA from Akabane virus with a detection limit of 11.5 copies of the virus,and had no cross reaction with bluetongue virus,bovine viral diarrhea virus and infectious bovine rhinotracheitis virus.The established RT-LAMP technique in… CONTINUE READING

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