Development of large DNA methods for plants: molecular cloning of large segments of Arabidopsis and carrot DNA into yeast.

@article{Guzmn1988DevelopmentOL,
  title={Development of large DNA methods for plants: molecular cloning of large segments of Arabidopsis and carrot DNA into yeast.},
  author={Pablo Guzm{\'a}n and Joseph R. Ecker},
  journal={Nucleic acids research},
  year={1988},
  volume={16 23},
  pages={11091-105}
}
Procedures for the preparation, analysis and cloning of large DNA molecules from two different plant species are described. Arabidopsis and carrot protoplasts were used for the preparation of large DNA molecules in agarose "plugs" or in solution. Pulsed-field gel electrophoresis (PFGE) analysis of large plant DNA preparations using a contour-clamped homogeneous field (CHEF) apparatus indicated that the size of the DNA was at least 12 Mb. Large DNA preparations were shown to be useful for… CONTINUE READING

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Advanced Bacterial Genetics (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY)

  • R. W. Davis, D. Botstein, J. R. Roth
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