Development of isotope labeling liquid chromatography mass spectrometry for mouse urine metabolomics: quantitative metabolomic study of transgenic mice related to Alzheimer's disease.

@article{Peng2014DevelopmentOI,
  title={Development of isotope labeling liquid chromatography mass spectrometry for mouse urine metabolomics: quantitative metabolomic study of transgenic mice related to Alzheimer's disease.},
  author={Jun Peng and Kevin Guo and Jianguo Xia and Jianjun Zhou and Jing Yang and David Westaway and David S. Wishart and Liang Li},
  journal={Journal of proteome research},
  year={2014},
  volume={13 10},
  pages={
          4457-69
        }
}
Because of a limited volume of urine that can be collected from a mouse, it is very difficult to apply the common strategy of using multiple analytical techniques to analyze the metabolites to increase the metabolome coverage for mouse urine metabolomics. We report an enabling method based on differential isotope labeling liquid chromatography mass spectrometry (LC-MS) for relative quantification of over 950 putative metabolites using 20 μL of urine as the starting material. The workflow… CONTINUE READING
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