Development of internal amplification controls for DNA profiling with the AmpFℓSTR(®) SGM Plus(®) kit.

@article{Zahra2011DevelopmentOI,
  title={Development of internal amplification controls for DNA profiling with the AmpFℓSTR(®) SGM Plus(®) kit.},
  author={Nathalie Zahra and Sibte Hadi and Judith Alexis Smith and Arati Iyengar and William Mark Goodwin},
  journal={Electrophoresis},
  year={2011},
  volume={32 11},
  pages={1371-8}
}
DNA extracted from forensic samples can be degraded and also contain co-extracted contaminants that inhibit PCR. The effects of DNA degradation and PCR inhibition are often indistinguishable when examining a DNA profile. Two internal amplification controls (IACs) were developed to improve quality control of PCR using the AmpFℓSTR® SGM Plus® kit. The co-amplification of these controls with DNA samples was used to monitor amplification efficiency and detect PCR inhibitors. IAC fragments of 90 and… CONTINUE READING