Development of high-speed ion conductance microscopy.

@article{Watanabe2019DevelopmentOH,
  title={Development of high-speed ion conductance microscopy.},
  author={Shinji Watanabe and Satoko Kitazawa and Linhao Sun and Noriyuki Kodera and Toshio Ando},
  journal={The Review of scientific instruments},
  year={2019},
  volume={90 12},
  pages={
          123704
        }
}
Scanning ion conductance microscopy (SICM) can image the surface topography of specimens in ionic solutions without mechanical probe-sample contact. This unique capability is advantageous for imaging fragile biological samples but its highest possible imaging rate is far lower than the level desired in biological studies. Here, we present the development of high-speed SICM. The fast imaging capability is attained by a fast Z-scanner with active vibration control and pipette probes with enhanced… 

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References

SHOWING 1-10 OF 65 REFERENCES

Characterization of AC mode scanning ion-conductance microscopy.

High-speed scanning ion conductance microscopy for sub-second topography imaging of live cells.

A high-speed SICM (HS-SICM) setup for topography imaging in the hopping mode with a pixel rate of 11.0 kHz is described, which is 15 times faster than what was reported before.

High Speed Scanning Ion Conductance Microscopy for Quantitative Analysis of Nanoscale Dynamics of Microvilli.

A new scanning algorithm is introduced for high speed SICM measurements using low capacitance and high-resonance-frequency piezo stages to characterize microvilli dynamics on surfaces, which shows clear structural changes after the epidermal growth factor stimulation.

Amplitude Modulation Mode of Scanning Ion Conductance Microscopy

An amplitude modulation (AM) mode of SICM is proposed, which employs an AC voltage to enhance the stability and improve the scanning speed and a capacitance compensation method to eliminate capacitance effect and use the amplitude of the AC current component to control the tip movement.

Lateral Resolution and Image Formation in Scanning Ion Conductance Microscopy.

Using microfabricated samples, the imaging of small cylindrical particles, elongated objects, and topography steps is investigated and the first direct comparison of numerical and experimental data is presented about the lateral resolution of the SICM.

Closed-loop ARS mode for scanning ion conductance microscopy with improved speed and stability for live cell imaging applications.

The results suggest that optimization of the tip approach speed can substantially improve S ICM imaging performance, further enabling SICM to become widely adopted as a general and versatile research tool for biological studies at the nanoscale level.

Multifunctional scanning ion conductance microscopy

The recent blossoming of SICM into a technique with a much greater diversity of applications and capability that can be used either standalone, with advanced control (potential–time) functions, or in tandem with other methods is highlighted.

The scanning ion-conductance microscope.

A scanning ion-conductance microscope (SICM) has been developed that can image the topography of nonconducting surfaces that are covered with electrolytes and sample and image the local ion currents above the surfaces.

Simultaneous scanning ion conductance and atomic force microscopy with a nanopore: Effect of the aperture edge on the ion current images

By integrating a nanopore onto the FluidFM, nanoscale features could be successfully imaged, but the increased sensitivity of the probe current to sample distance comes with higher sensitivity to an inherent SICM wall artefact.

Alternative configuration scheme for signal amplification with scanning ion conductance microscopy.

This work reports the development of an alternative configuration scheme for SICM signal amplification that is based on applying current to the nanopipette and demonstrates successful imaging of L929 fibroblast cells and discusses the capabilities of this instrument configuration for future applications.
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