Development of hemoglobin A1c certified reference material by liquid chromatography isotope dilution mass spectrometry.

@article{Bi2012DevelopmentOH,
  title={Development of hemoglobin A1c certified reference material by liquid chromatography isotope dilution mass spectrometry.},
  author={Jiaming Bi and Liqing Wu and Bin Yang and Yi Yang and Jing Wang},
  journal={Analytical and bioanalytical chemistry},
  year={2012},
  volume={403 2},
  pages={549-54}
}
We report the development of a National Institute of Metrology (NIM) hemoglobin A(1c) (HbA(1c)) certified reference material (CRM). Each CRM unit contains about 10 μL of hemoglobin. Both hemoglobin and glycated hemoglobin were quantitatively determined by high-performance liquid chromatography (HPLC)-isotope dilution mass spectrometry (IDMS) with synthesized VHLTPE and glycated VHLTPE as standards. The mass fraction of synthesized VHLTPE or glycated VHLTPE was also quantitatively determined by… CONTINUE READING