Development of gene expression system in a marine diatom using viral promoters of a wide variety of origin.

  title={Development of gene expression system in a marine diatom using viral promoters of a wide variety of origin.},
  author={Kunihiro Sakaue and Hisashi Harada and Yusuke Matsuda},
  journal={Physiologia plantarum},
  volume={133 1},
Promoter sequences of the cytomegalovirus (PCMV), the rous sarcoma virus long terminal repeat (PRSV-LTR) and the cauliflower mosaic virus 35s (PCaMV35s) were ligated with the beta-glucuronidase (GUS) gene, uidA, and were introduced into cells of the marine diatom, Phaeodactylum tricornutum. Transformants were selected on a 100 mg l(-1) Zeocin plate, and Zeocin-resistant clones were further selected by the occurrence of GUS activity. Two to 10 GUS-positive clones were obtained, and GUS… CONTINUE READING
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