Development of fructosyl amine oxidase specific to fructosyl valine by site-directed mutagenesis.

@article{Miura2008DevelopmentOF,
  title={Development of fructosyl amine oxidase specific to fructosyl valine by site-directed mutagenesis.},
  author={Seiji Miura and Stefano Ferri and Wakako Tsugawa and Seungsu Kim and Koji Sode},
  journal={Protein engineering, design & selection : PEDS},
  year={2008},
  volume={21 4},
  pages={233-9}
}
Docking models of fructosyl amine oxidase (FAOD) from the marine yeast Pichia N1-1 (N1-1 FAOD) with the substrates fructosyl valine (f-Val) and fructosyl-(epsilon)N-lysine (f-(epsilon)Lys) were produced using three-dimensional protein model as reported previously (Miura et al., 2006, Biotechnol. Lett., 28, 1895-1900). The residues involved in recognition of substrates were proposed, particularly Asn354, which interacts closely with f-(epsilon)Lys, but not with f-Val. Substitution of Asn354 to… CONTINUE READING

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