Development of effective cross-linking method for bioactive substance--enzyme immobilization using glutaraldehyde oligomers.

@article{Nakagawa1989DevelopmentOE,
  title={Development of effective cross-linking method for bioactive substance--enzyme immobilization using glutaraldehyde oligomers.},
  author={Terumichi Nakagawa and Kazuma Izawa and Shintaro Yagi and Akimasa Shibukawa and Hirotaka Tanaka and Toshihiko Tashima and Masanori Imai},
  journal={Chemical & pharmaceutical bulletin},
  year={1989},
  volume={37 9},
  pages={2463-6}
}
When a 10% aqueous solution of glutaraldehyde (GA) was alkalized to pH 8.5 in borate buffer solution and heated at 60 degrees C, the ultraviolet spectrum of GA solution showed two distinct absorption maxima. The one at 280 nm with a weak absorbance ascribable to the C = O bond in the aldehyde group shifted to near 300 nm after 50 min with a slight increase… CONTINUE READING