Development of digoxigenin-labeled PCR amplicon probes for use in the detection and identification of enteropathogenic Yersinia and Shiga toxin-producing Escherichia coli from foods.

@article{Weagant1999DevelopmentOD,
  title={Development of digoxigenin-labeled PCR amplicon probes for use in the detection and identification of enteropathogenic Yersinia and Shiga toxin-producing Escherichia coli from foods.},
  author={Stephen D. Weagant and J A Jagow and Karen C. Jinneman and Curtis John Omiecinski and Charles A. Kaysner and Walter E. Hill},
  journal={Journal of food protection},
  year={1999},
  volume={62 5},
  pages={438-43}
}
By including digoxigenin-11-dUTP in a polymerase chain reaction (PCR), amplification products were produced that contained nonisotopic markers for use as DNA hybridization probes. Because these labeled amplicons encode pathogenic traits for specific foodborne bacteria, they can be used to detect the presence of potentially virulent organisms that may be present in foods. This technology allows the synthesis of a variety of shelf-stable probe reagents for detecting a number of foodborne microbes… CONTINUE READING