Development of conventional and real-time PCR assays for detection of Legionella DNA in respiratory specimens.

Abstract

The development and validation of a PCR assay based on the use of new 16S ribosomal DNA (rDNA)-targeted primers to detect Legionella DNA in respiratory specimens are described. The assay was originally developed as conventional PCR followed by electrophoretic detection and was then adapted to Lightcycler format with SYBR Green I detection and melting curve… (More)

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@article{RantakokkoJalava2001DevelopmentOC, title={Development of conventional and real-time PCR assays for detection of Legionella DNA in respiratory specimens.}, author={Kaisu Rantakokko-Jalava and Jari Jalava}, journal={Journal of clinical microbiology}, year={2001}, volume={39 8}, pages={2904-10} }