Development of conventional and nested PCR assays for the detection of Ophiocordyceps sinensis.

  title={Development of conventional and nested PCR assays for the detection of Ophiocordyceps sinensis.},
  author={Guo-Sheng Jin and Xiao-Liang Wang and Yi Li and Wen-jing Wang and Rui-Heng Yang and Shu-Yu Ren and Yi-Jian Yao},
  journal={Journal of basic microbiology},
  volume={53 4},
Ophiocordyceps sinensis, endemic to the Tibetan Plateau, is one of the most important medicinal fungi with a huge economic value. In the present study, specific primer pairs were designed based on a comprehensive ITS sequence dataset of O. sinensis and its related fungi, and tested for specificity and sensitivity through PCR experiments using 27 individuals of O. sinensis from different geographical origins and 40 other related fungal species in terms of phylogeny or ecology. A primer pair… CONTINUE READING
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