Development of cloned DNA probes for the identification of snail intermediate hosts within the genus Bulinus.

@article{Strahan1990DevelopmentOC,
  title={Development of cloned DNA probes for the identification of snail intermediate hosts within the genus Bulinus.},
  author={K Strahan and Richard A. Kane and David Rollinson},
  journal={Acta tropica},
  year={1990},
  volume={48 2},
  pages={117-26}
}
DNA fragments have been isolated from Bulinus cernicus and cloned in Escherichia coli strain JM109 using pUC18 as the vector. Four recombinant plasmids designated pBC123, pBC126, pBC127 and pBC134 were selected for further analysis and were shown by both dot and Southern blot hybridisations to have different specificities to species representing the four species groups of Bulinus. Non-specific hybridisation occurred with pBC126 whereas pBC123 and pBC134 hybridised only to B. cernicus. Partial… CONTINUE READING