Development of ascorbate transporters in brain cortical capillary endothelial cells in culture

@article{Qiao2008DevelopmentOA,
  title={Development of ascorbate transporters in brain cortical capillary endothelial cells in culture},
  author={H. Qiao and J. M. May},
  journal={Brain Research},
  year={2008},
  volume={1208},
  pages={79-86}
}
Ascorbic acid in its reduced form is not transported across the capillary endothelial cell blood-brain barrier. This is thought to be due to absence of the SVCT2, a specific transporter for ascorbate. To assess this directly we prepared primary cultures of mouse cortical microvascular endothelial cells. When still in the capillaries, these cells did not express the SVCT2 protein as assessed by immunocytochemistry and by immunoblotting. However, during several days in culture, they developed… Expand
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