Development of array-based technology for detection of HAV using gold-DNA probes.

@article{Wan2005DevelopmentOA,
  title={Development of array-based technology for detection of HAV using gold-DNA probes.},
  author={Zhixiang Wan and Yefu Wang and Shawn Shun-cheng Li and Lianlian Duan and Jianxin Zhai},
  journal={Journal of biochemistry and molecular biology},
  year={2005},
  volume={38 4},
  pages={
          399-406
        }
}
A sensitive method for detection of Hepatitis A virus (HAV) by utilizing gold-DNA probe on an array was developed. Amino-modified oligodeoxynucleotides at the 5' position were arrayed on an activated glass surface to function as capture probes. Sandwich hybridization occurred among capture probes, the HAV amplicon, and gold nanoparticlesupported oligonucleotide probes. After a silver enhancement step, signals were detected by a standard flatbed scanner or just by naked eyes. As little as 100 fM… CONTINUE READING

Topics from this paper.

Citations

Publications citing this paper.

References

Publications referenced by this paper.
SHOWING 1-10 OF 23 REFERENCES

Single particle sensitivity in the optical detection of individual DNA - conjugated nanoparticle after metal enhancement

A. Csaki, P. Kaplanek, R. Möller, W. Fritzsche
  • Nanotechnology
  • 2003
VIEW 1 EXCERPT

Single particle sensitivity in the optical detection of individual DNA-conjugated nanoparticle after metal

A. Csaki, P. Kaplanek, R. Möller, W. Fritzsche
  • enhancement. Nanotechnology
  • 2003
VIEW 1 EXCERPT

Electrochemical detection of DNA hybridization based on silver - enhanced gold nanoparticle label

C. Hong, W. Yanqing, H. Pingang, F. Yuzhi
  • Anal . Chim . Acta .
  • 2002
VIEW 1 EXCERPT

Optical Detection of DNA constructs based on nanoparticles and silver enhancement

Z. Guo-Jun, M. Robert, C. Andrea, F. Wolfgang
  • AIP Conference Proceedings
  • 2002