Development of antisera selective for m4 and m5 muscarinic cholinergic receptors: distribution of m4 and m5 receptors in rat brain.

@article{Yasuda1993DevelopmentOA,
  title={Development of antisera selective for m4 and m5 muscarinic cholinergic receptors: distribution of m4 and m5 receptors in rat brain.},
  author={Robert P. Yasuda and Włodzimierz Cieśla and Lauren R. Flores and Stephen J Wall and Mika Li and S A Satkus and Jason S Weisstein and B V Spagnola and Barry B. Wolfe},
  journal={Molecular pharmacology},
  year={1993},
  volume={43 2},
  pages={149-57}
}
A portion of the cDNA sequence corresponding to the third intracellular loop of either the m4 or m5 muscarinic cholinergic receptor was ligated into the pRIT23 or pET-3a expression vector, respectively. The expressed fusion proteins were purified and used to develop selective polyclonal antisera to the m4 and m5 muscarinic receptors. These antisera were used in an immunoprecipitation protocol to examine quantitatively the distribution of receptor subtypes in regions of rat brain. The density of… CONTINUE READING
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