Development of anonymous nuclear markers from Illumina paired-end data for Seychelles caecilian amphibians (Gymnophiona: Indotyphlidae)

@article{Lewis2013DevelopmentOA,
  title={Development of anonymous nuclear markers from Illumina paired-end data for Seychelles caecilian amphibians (Gymnophiona: Indotyphlidae)},
  author={Claire J. Lewis and Simon T. Maddock and Julia J Day and Ronald Nussbaum and Charles Morel and Mark Wilkinson and Peter G Foster and David J Gower},
  journal={Conservation Genetics Resources},
  year={2013},
  volume={6},
  pages={289-291}
}
Anonymous nuclear markers were developed for Seychelles caecilian amphibians. Using a previously published bioinformatics pipeline (developed for Roche 454 data), 36 candidate anonymous nuclear loci (ANL) of at least 180 bp length were identified from Illumina MiSeq next generation sequencing data for five Seychelles species. We designed primer pairs for the 36 candidate ANL and tested these by PCR and Sanger sequencing. Seven ANL amplified and sequenced well for at least five of the six… CONTINUE READING
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References

Publications referenced by this paper.
Showing 1-10 of 22 references

A ninefamily classification of caecilians (Amphibia: Gymnophiona)

  • M Wilkinson, D San Mauro, E Sherratt, DJ Gower
  • Zootaxa
  • 2011
1 Excerpt

Caecilians (Gymnophiona)

  • DJ Gower, M Wilkinson
  • Conservation International and NatureServe,
  • 2008
1 Excerpt

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