Development of anAgrobacterium-mediated transformation system for regenerating garland chrysanthemum (Chrysanthemum coronarium L.)

@article{Chung2009DevelopmentOA,
  title={Development of anAgrobacterium-mediated transformation system for regenerating garland chrysanthemum (Chrysanthemum coronarium L.)},
  author={Kwi-Mi Chung and Young-Doo Park},
  journal={Journal of Plant Biology},
  year={2009},
  volume={48},
  pages={136-141}
}
Although efficient shoot regeneration and selection are essential for genetic transformation mediated byAgrobacterium, success has been limited with the garland chrysanthemum (Chrysanthemum coronarium L.). In this study, we developed a useful protocol for shoot regeneration with leaf disk explants. The optimal concentrations of NAA and BA were 0.2 mg L−1 and 0.5 mg L−1, respectively. To optimize the selection system for regenerating plants from genetically transformed tissues, we tested the… CONTINUE READING