Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge.

@article{Wagner1994DevelopmentOA,
  title={Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge.},
  author={Michael S. Wagner and R. Erhart and Werner Manz and Rudolf I Amann and Hilde Lemmer and D Wedi and Karl Heinz Schleifer},
  journal={Applied and environmental microbiology},
  year={1994},
  volume={60 3},
  pages={792-800}
}
Enhanced biological phosphate removal in an anaerobic-aerobic activated sludge system has generally been ascribed to members of the genus Acinetobacter. A genus-specific 16S rRNA-targeted oligonucleotide probe was developed to investigate the role of Acinetobacter spp. in situ. Nonisotopic dot blot hybridization to 66 reference strains, including the seven described Acinetobacter spp., demonstrated the expected probe specificity. Fluorescent derivatives were used for in situ monitoring of… CONTINUE READING
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